The magic "uh-oh" number of carries for an RB is 370. Please note that this only includes carries, and not "touches" (i.e. rushes and receptions). Also, there are other risk factors to look out for. A sudden uncharacteristic rise in ypc is almost always a very very bad sign, for instance.
Read
this article for statistics and details.
One last note: that article is 2 years old. It marked Ricky Williams, Jamal Lewis, LaDainian Tomlinson, and Ahman Green as guys to watch out for in 2004. How did they do?
LaDainian Tomlinson lost almost 600 combined yards from the previous season, although his TDs stayed more or less the same. Most alarming of all, he battled injuries all season, and saw his ypc drop by *1.4*. That's HUGE. Total losses included almost 60 fantasy points and over 70 points of VBD.
Jamal Lewis lost well over 1000 combined yards from the previous season, as well as 50% of his TDs. He missed 4 games due to injury and saw his ypc drop by a full yard. He put up less than HALF as many fantasy points in 2004 as in 2003, and his VBD dropped from 169 to ZERO.
Ahman Green lost about 800 combined yards and 12 TDs. Unlike Tomlinson and Lewis, Green stayed pretty healthy, missing only a single game... but he still saw his ypc drop by .8. His fantasy points were reduced by 150, and his VBD went from 203 to 39.
Ricky Williams... well, as the article stated, Ricky Williams retired, citing ridiculous workload as one of the reasons.